LOCUS       pDONR™/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1607..1703)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1718..1737)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1745..1761
                     /note="M13 Reverse priming site"
     gene            1874..2683
                     /note="Kanamycin resistance gene"
     rep_origin      2804..3477
                     /note="pUC origin"
     vector          join(1615..3480,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1600
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:51901 IMAGE:5769575"
                     /tissue_type="Brain, fetal, whole pooled"
                     /clone_lib="NIH_MGC_121"
                     /lab_host="DH10B"
                     /note="Vector: pCMV-SPORT6"
     gene            692..1600
                     /gene="ST3GAL3"
                     /gene_synonym=ST3Gal III
                     /gene_synonym=ST3GALII
                     /gene_synonym=ST3GalIII
                     /gene_synonym=ST3N
                     /db_xref="GeneID:6487"
                     /db_xref="HGNC:10866"
                     /db_xref="MIM:606494"
     CDS             692..1600
                     /dnas_title="ST3 beta-galactoside
                     alpha-2,3-sialyltransferase 3"
                     /gene="ST3GAL3"
                     /gene_synonym=ST3Gal III
                     /gene_synonym=ST3GALII
                     /gene_synonym=ST3GalIII
                     /gene_synonym=ST3N
                     /codon_start=1
                     /product="ST3 beta-galactoside
                     alpha-2,3-sialyltransferase 3"
                     /protein_id="AAH50380.1"
                     /db_xref="GI:29791922"
                     /db_xref="GeneID:6487"
                     /db_xref="HGNC:10866"
                     /db_xref="MIM:606494"
                     /translation="MGLLVFVRNLLLALCLFLVLGFLYYSAWKLHLLQWEEDSNSVVL
                     SFDSAGQTLGSEYDRLGFLLNLDSKLPAELATKYANFSEGACKPGYASALMTAIFPRF
                     SKPAPMFLDDSFRKWARIREFVPPFGIKGQDNLIKAILSVTKEYRLTPALDSLRCRRC
                     IIVGNGGVLANKSLGSRIDDYDIVVRLNSAPVKGFEKDVGSKTTLRITYPEGAMQRPE
                     QYERDSLFVLAGFKWQDFKWLKYIVYKERVSASDGFWKSVATRVPKEPPEIRILNPYF
                     IQEAAFTLIGLPFNNGLMGRGNIPTLGSVAVTMALHGCDEVAVAGFGYDMSTPNAPLH
                     YYETVRMAAIKESWTHNIQREKEFLRKLVKARVITDLSSGI"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cgaattagcc accaagtacg caaacttttc
        721 agagggagct tgcaagcctg gctatgcttc agccttgatg acggccatct tcccccggtt
        781 ctccaagcca gcacccatgt tcctggatga ctcctttcgc aagtgggcta gaatccggga
        841 gttcgtgccg ccttttggga tcaaaggtca agacaatctg atcaaagcca tcttgtcagt
        901 caccaaagag taccgcctga cccctgcctt ggacagcctc cgctgccgcc gctgcatcat
        961 cgtgggcaat ggaggcgttc ttgccaacaa gtctctgggg tcacgaattg acgactatga
       1021 cattgtggtg agactgaatt cagcaccagt gaaaggcttt gagaaggacg tgggcagcaa
       1081 aacgacactg cgcatcacct accccgaggg cgccatgcag cggcctgagc agtacgagcg
       1141 cgattctctc tttgtcctcg ccggcttcaa gtggcaggac tttaagtggt tgaaatacat
       1201 cgtctacaag gagagagtga gtgcatcgga tggcttctgg aaatctgtgg ccactcgagt
       1261 gcccaaggag ccccctgaga ttcgaatcct caacccatat ttcatccagg aggccgcctt
       1321 caccctcatt ggcctgccct tcaacaatgg cctcatgggc cgggggaaca tccctaccct
       1381 tggcagtgtg gcagtgacca tggcactaca cggctgtgac gaggtggcag tcgcaggatt
       1441 tggctatgac atgagcacac ccaacgcacc cctgcactac tatgagaccg ttcgcatggc
       1501 agccatcaaa gagtcctgga cgcacaatat ccagcgagag aaagagtttc tgcggaagct
       1561 ggtgaaagct cgcgtcatca ctgatctaag cagtggcatc TAGGACCCAG CTTTCTTGTA
       1621 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT
       1681 CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA
       1741 CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT
       1801 TGCACAAGAT AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA
       1861 TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA
       1921 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC
       1981 GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA
       2041 AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT
       2101 TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC
       2161 CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA
       2221 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA
       2281 TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA
       2341 CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT
       2401 CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA
       2461 TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG
       2521 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA
       2581 GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT
       2641 GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG
       2701 GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA
       2761 ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA
       2821 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC
       2881 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT
       2941 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG
       3001 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC
       3061 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT
       3121 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA
       3181 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT
       3241 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG
       3301 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA
       3361 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA
       3421 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT
//